The Massive Impact of Ram's Sperm Starvation on the Fertilization and Blastocyst Rates in Terms of Sperm Quality and Capacitation

To obtain a high in vitro fertilization rate while ensuring the ideal development of the
 embryos, thus eliminating most critical problems assisted reproductive
 technology (ART) (implantation and low pregnancy rates). Therefore, sperm taken
 from caudal epididymis must undergo acquisition progressive motility and
 fertilization. This process takes place through series physiological biochemical
 changes, known as capacitation, prerequisite for In this work, we have
 developed new way to incubate using four different treatments terms of
 energy substrates incubation methods. restriction recovery (RSE) treatment, after starving 50 min until they lost their capacity
 motility, were given glucose incubated 60 min. As other
 treatments, not exposed starvation by standard methods maturation capacitation sperm, but with energy
 substrates. According results obtained, was hyperactive showed a
 significant increase ability fertilize oocytes treatment that involved
 then saving adding glucose, compared other treatments.
 conclusion, effects feature persisted fertilization, which led to
 production percentage blastocysts, well it has found starvation
 rescue are adequate fertilizing developing embryos sheep.